The United Nations-backed Sport Positive has been ranking the environmental efforts of all 20 of England's top-flight teams for the past four years.
Clubs were scored on 12 categories ranging from active efforts against climate change, policies and education on the matter.
For the fourth consecutive year, Spurs have achieved top spot with the club's education on sustainability across its teams as one of the main successes.
The club's Executive Director, Donna-Maria Cullen, said: "We are proud to have been named at the top of the Sport Positive league table for a fourth year running.
"As a Club, we are acutely aware of the role we can play in addressing the threat posed by climate change and have embedded environmental sustainability within everything we do.
"It is extremely important we take our players with us on this journey and the first-of-its-kind training we have recently facilitated for all teams is a huge part of this.
"We also acknowledge there is still so much more to do as we work towards achieving our net zero target of 2040."
Welsh defender Ben Davies said: "As players we’re fully aware of the amount of work the Club is doing on the sustainability front.
"The training gave us an understanding of the impact a huge organisation like ours can have on the environment while also talking us through the ways we can reduce our emissions and be as sustainable as possible.
"The more educated we are on the subject, the better we can be – it’s about taking small steps as individuals and, by using our platforms to encourage others to do the same, it all adds up."
The north London side has been joined by Liverpool for the second year running with the Reds' focus on policy and commitment featuring as their strong point in the division."
Premier League leaders Manchester City were narrowly behind Liverpool in third with rock-bottom Southampton taking fourth.
