She underwent surgery after rupturing her anterior cruciate ligament during Arsenal's 1-0 Champions League group-stage defeat to Olympique Lyonnais.
The World Cup will be held in Australia and New Zealand from July 20th to August 20th. The Netherlands finished runners-up at the 2019 edition in France.
"No hopes. I'm definitely going to miss the World Cup which is a hard one," Miedema told the BBC on Sunday.
"I'm hoping to be back for the beginning of next season. That's my focus right now - I need to be realistic. Recovery is going really well. It's slow but little steps."
Miedema, who has 95 international goals in 115 appearances, was part of the Netherlands side that won the 2017 European Championship on home soil.