Mitchell out as Borthwick names England squad for Autumn internationals
Mitchell has not played this season because of a neck injury and Northampton have been unable to provide a timeframe for his return.
George Ford was also left out of Borthwick's 36-man squad as he battles a thigh issue, but the Sale fly-half will remain with the group that departs for a training camp in Spain on Monday to continue his rehabilitation.
Fraser Dingwall is another notable absence due to injury, but Henry Slade is included despite only just returning from shoulder surgery.
England host New Zealand, Australia, South Africa and Japan in November.
"Naming this squad is an exciting step in our preparations for the Autumn Nations Series and we look forward to working with the players again in the days ahead," said Borthwick.
"Facing New Zealand in the opening match is a tremendous opportunity for us to test ourselves against one of the best teams in the world."