Saturday's game was the first action in the league since the injury that led to the Bengals-Bills game being abandoned.
The 24-year-old Hamlin suffered cardiac arrest in the Bills' Monday night NFL game against the Bengals in Cincinnati.
He collapsed after taking a hard hit to the chest while tackling Bengals receiver Tee Higgins during the first quarter of the nationally televised game.
Hamlin was resuscitated on the field and hospitalized in critical condition at the University of Cincinnati Medical Center (UCMC).
The player had a breathing tube removed on Friday and was able to hold a video call with team-mates.
Players and coaching staff at Las Vegas' Allegiant Stadium, wore tee-shirts with 'Love for 3 Damar' while Chiefs quarterback Patrick Mahomes wore a hoodie with the slogan 'Hamlin Strong' on the back and an image of the player.
Before the national anthem, the crowd were urged to show their support for Hamlin with cheers and applause.
The Bills released an update on Hamlin on Saturday.
"Per the physicians at UC Medical Center, Damar is making continued progress in his recovery yet remains in critical condition. He continues to breathe on his own and his neurological function is excellent," the team said.
