Kinghorn returns to Scotland side for Six Nations clash with England
Scotland coach Gregor Townsend has also selected Kyle Steyn and former captain Jamie Ritchie in his starting XV following an agonising 20-16 loss to France in the second round.
Kyle Rowe, Harry Paterson and Matt Fagerson - who all started against Les Bleus - have been dropped from the matchday 23 entirely, with the bench unchanged.
Full-back Kinghorn missed the opening two matches of the Six Nations through injury, while wing Steyn was a late dropout against France after his wife went into labour.
Blindside flanker Ritchie returns to the back row after starting in Scotland's dramatic 27-26 first-round victory over Wales in Cardiff.
Scotland are bidding for a fourth successive win over oldest rivals England, who are due to name their team later Thursday.
Scotland (15-1):
Blair Kinghorn; Kyle Steyn, Huw Jones, Sione Tuipulotu, Duhan van der Merwe; Finn Russell (co-capt), Ben White; Jack Dempsey, Rory Darge (co-capt), Jamie Ritchie; Scott Cummings, Grant Gilchrist; Zander Fagerson, George Turner, Pierre Schoeman
Replacements: Ewan Ashman, Alec Hepburn, Elliot Millar-Mills, Sam Skinner, Andy Christie, George Horne, Ben Healy, Cameron Redpath
Coach: Gregor Townsend (SCO)