Murray confirms withdrawal from men's singles at Wimbledon
Two-time Wimbledon champion Murray's involvement in the tournament had been in doubt after undergoing back surgery 10 days ago.
He had been due to face Tomas Machac of the Czech Republic on Centre Court later on Tuesday.
Murray had been practising for the match on Monday but delayed a decision to play to give him the best possible chance of participating.
"Unfortunately, despite working incredibly hard on his recovery since his operation just over a week ago, Andy has taken the very difficult decision not to play the singles this year," a statement from Murray's team read.
"As you can imagine, he is extremely disappointed but has confirmed that he will be playing in the doubles with Jamie and looks forward to competing at Wimbledon for the last time."
Murray suffered a back injury in the Wimbledon warm-up at Queen's, forcing him to retire during his match against Australia's Jordan Thompson.
The 37-year-old underwent surgery to remove a spinal cyst which had been causing him pain in his right leg, severely hampering his chances of making the grass-court Grand Slam.
Murray will now negotiate the doubles tournament with brother Jamie before turning his attention to the 2024 Olympics in Paris this summer.
He has been named to play in the singles and doubles alongside Dan Evans on the clay at Roland Garros.