Murray's family question absence of ex-Wimbledon champion from poster
The All England Club posted an image of 15 famous players walking down a staircase on its social media channels.
Under the words "Here's to the next generation of headline acts", young players Jannik Sinner and Carlos Alcaraz - neither of whom have made an impact at the championships yet - stand in the foreground.
Behind them are a selection of "epic" rivalries including Roger Federer, Novak Djokovic and Rafael Nadal, Bjorn Borg and John McEnroe, Chris Evert and Martina Navratilova and Serena and Venus Williams.
But Murray, who ended Britain's 77-year wait for a men's champion in 2013, is nowhere to be seen.
His uncle, Niall Erskine, tweeted underneath the image: "Appalling at every level, all about the men in the forefront and your own British history-maker nowhere to be seen. You should be ashamed of yourselves."
And Jamie Murray, who has been competing along with his brother at Queen's Club this week, replied to Wimbledon's Instagram post with "Where's @andymurray?"
He added: "Didn't they talk about the big 4 for 10 years until he smashed his hip to pieces when he was world No 1?"
Illustrator Grant Gruenhaupt responded by saying more paintings are planned.
Jamie Murray and partner Michael Venus lost their first-round doubles match at Queen's against Matthew Ebden and Rohan Bopanna in two tie-breaks.
Andy Murray, currently ranked 38th in singles, was due to play in the doubles alongside Cameron Norrie later on Wednesday.
But the Scot, knocked out of the singles on Tuesday, has withdrawn from the event.